Research - Laboratory/Non-Laboratory, Staff/Administrative
Working at MIT offers opportunities, an environment, a culture - and benefits - that just aren't found together anywhere else. If you're curious, motivated, want to be part of a unique community, and help shape the future - then take a look at this opportunity.
RESEARCH SUPPORT ASSOCIATE 2, Koch Institute for Integrative Cancer Research-Anderson Lab , to participate in developing novel nucleic acid-based therapeutics in a lab focused on the interface of biotechnology and materials science. Responsibilities include formulating lipid and polymeric RNA nanoparticles; characterizing nanoparticles through dynamic light scattering, RiboGreen assays, and other techniques; evaluating efficacy of mRNA nanoparticles in vitro using plate-based bioluminescence/fluorescence assays, microscopy, and flow cytometry; evaluating efficacy of mRNA nanoparticles in vivo (will involve handling/injecting mice, whole animal/organ imaging, dissections, tissue processing for protein, DNA, and RNA extraction, and flow cytometry of dissociated tissue); synthesizing mRNA through in vitro transcription; fabricating PDMS microfluidic mixing devices for nanoparticle formulation; assisting with managing inventory of RNA and lipid stocks and other reagents used for formulation/transfection; and other duties as needed.
REQUIRED : high school diploma or its equivalent; willingness to work with animals and learn new tools/techniques; strong organizational, interpersonal, and communication skills; ability to work independently and as part of a multidisciplinary team; comfort summarizing and reporting results to researchers; meticulous attention to detail; practiced documentation skills; awareness of basic safety issues, following safety procedures, and maintaining a safe work environment; good judgement with confidential information/issues; and experience with flow cytometry, flow cytometry analysis software, ELISA, PDMS device fabrication, mRNA synthesis and characterization, and primary cell isolation. PREFERRED : B.S. in biology, biochemistry, chemistry, chemical engineering, biological engineering, or related field; one year of specialized lab experience (including that gained as an undergraduate); experience in at least one of the following--mouse handling and injections, nanoparticle formulation and characterization, passaging and transfecting cultured cells, RNA and protein expression analysis, and molecular cloning. Must be willing to work flexible hours to accommodate cell culture and sampling. Job 17791-4
This is a one-year term-limit appointment, with the possibility of renewal based upon funding availability.
Internal Number: 17791
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